IC50 values are presented as the means ?SDs (n=4) and * denotes p<0.05. Following 72h treatment, cells were subject to a resazurin reduction assay. Voreloxin is a potent Topoisomerase II inhibitor with broad-spectrum anti-tumor activity. Data shown are representative of three independent experiments.
Daunorubicin is a topoisomerase II inhibitor that induces apoptosis.
After 24 hours, complete culture medium was changed into fresh low-serum-containing medium (1% FBS) containing DMSO (control) or indicated doses of Irinotecan (Selleckchem). All Rights Reserved. Another strand (in case of topoisomerase I) or another double helix (in case of topoisomerase II) passed through the broken end. Antineoplastic and Immunosuppressive Antibiotics, Selection Antibiotics for Transfected Cell, FDA-approved & Passed Phase I Drug Library, Human Endogenous Metabolite Compound Library, Anti-cancer Compound Library (Verified by Broad Institute), Anti-Cardiovascular Disease Compound Library, Cytoskeletal Signaling Pathway Compound Library, Endoplasmic Reticulum Stress Compound Library, Protein-protein Interaction Inhibitor Library, Small Molecule Immuno-Oncology Compound Library. 9-amino-CPT (9-Aminocamptothecin) is a Topoisomerase I inhibitor with potent anticancer activities. Gatifloxacin (AM-1155, CG5501, BMS-206584) is an antibiotic of the fourth-generation fluoroquinolone family, and inhibits the bacterial enzymes DNA gyrase and topoisomerase IV. Etoposide induces autophagy, mitophagy and apoptosis.
A black female mouse was crossed with a black male mouse, 3 of their 10 offspring were white. Phase 3. Genz-644282 can be used in cancer research. Here is part of the Wikipedia entry - does any of this help? It has selective inhibitory effect on the phosphorylation of histone H1 and H3, but less effect on other histones. Growth sensitivity of HEY cells in the presence of Camptothecin(CPT) was monitored using clonogenic assay.
Beta-Lapachone is a selective DNA topoisomerase I inhibitor, exhibiting no inhibitory activities against DNA topoisomerase II or ligase. Pax3:Foxo1a knockdown increases select chemotherapy sensitivities. Type I topoisomerase cuts one strand of a DNA double helix and then reanneals the cut strand. SW-044248 is a non-canonical Top1 inhibitor with a pattern of selective toxicity for NSCLC cells. 10-Hydroxycamptothecin is a cell-permeable powerful DNA topoisomerase I inhibitor. i am studying molecular biology so details are greatly appreciated.
Type II is a 174 kDa homodimer. Parasites were metabolically labeled with [3H]hypoxanthine, treated with etoposide (■), gatifloxacin (●), levofloxacin(▲), ciprofloxacin (□), fleroxacin (○), or nalidixic acid (△) at the indicated concentrations for 18-22 h, lysed with SDS and digested with RNases. Berberine chloride induces apoptosis with sustained phosphorylation of JNK and p38 MAPK, as well as generation of the ROS. Betulinic acid (ALS-357), a pentacyclic triterpenoid from Syzigium claviflorum, is a inhibitor of HIV-1 with EC50 of 1.4 μ M. Betulinic acid acts as a new activator of NF-kB.Phase 1/2. Fluorescence micrographs of mammosphere cells after incubated with the simple mixture of free DOX&CPT, PFP-DC nanoparticles, and HAC-PFPDC nanoparticles for 3 h at 37℃. Students t-test. Cell viability 48 hours after treatment was determined by AlamarBlue assay (Invitrogen) according to manufacturer's instructions. Coefficient of variation for each data point was 19%. Daunorubicin HCl (Daunomycin) inhibits both DNA and RNA synthesis and inhibits DNA synthesis with Ki of 0.02 μM in a cell-free assay. Panc1 cells were plated in triplicates into 48-well plates at a density of 10,000 cells/ml. Ciprofloxacin (Bay o 9867) is a fluoroquinolone antibiotic, shows broad and potent antibacterial activity, with MIC90 of 0.024-6 μM. Completing the CAPTCHA proves you are a human and gives you temporary access to the web property. • 7-Ethyl Camptothecin is an anti-cancer chemical that exhibits a strong activity against various murine tumors through Topo I (topoisomerase) inhibition.
Will cows evolve to be intelligent like humans? A) MV411 or B) Kasumi 1 cells were incubated with YM155 (0.25 x IC50) in the presence or absence of i) 0.5 x IC50 or ii) 1 x IC50 of Daunorubicin or iii) 0.5 x IC50 or iv) 1 x IC50 of Cytarabine for 72h.
Cell lysates were then subjected to Western blot analysis.
Cell viabilities with increasing concentrations of cisplatin (CP) and doxorubicin (DOXO) under normoxic and hypoxic condition for 48 hours were determined by MTT assay. © Copyright 2013 Selleck Chemicals. What is a bidirectional replication? Irinotecan is a topoisomerase I inhibitor for LoVo cells and HT-29 cells with IC50 of 15.8 μM and 5.17 μM, respectively. Cell viability (A) and caspase 3/7 activity (B) was determined after treatment with ZA (zoledronic acid), RIS (risedronate), IBN (ibandronate), ALN (alendronate) in combination with carbenoxolone, novobiocin and ibrutinib. What Is A Bidirectional Replication? Cell viability 72 hours after treatment was determined by AlamarBlue assay (Invitrogen) according to manufacturer's instructions. *p < 0.05. The fold increase in nuclear DNA capture as compared to solvent controls (mean of triplicate determinations) was plotted against concentration. Nalidixic acid (NSC-82174) is a synthetic 1,8-naphthyridine antimicrobial agent with a limited bacteriocidal spectrum. Moxifloxacin (BAY12-8039) is a fourth-generation synthetic fluoroquinolone antibacterial agent. Enoxacin (AT-2266, CI919, Pd107779, NSC 629661) is an oral broad-spectrum fluoroquinolone antibacterial agent by inhibiting bacterial DNA gyrase and topoisomerase IV, used to treat a wide variety of infections. It acts by inhibiting DNA gyrase (bacterial topoisomerase II). Camptothecin (NSC-100880, CPT) is a specific inhibitor of DNA topoisomerase I (Topo I) with IC50 of 0.68 μM in a cell-free assay. Ellipticine Hydrochloride is a natural product isolated from the Australian evergreen tree of the Apocynaceae family with antineoplastic activity.
1: creates single strand nick, rotates the DNA to unwind and loosen and then reseals it 2: double strand nicks in the DNA and passes the DNA strand through the nick and reseals the nick. Genz-644282 is a non-camptothecin topoisomerase I (Topo I) inhibitor that demonstrates potent cytotoxic activity with a median IC50 of 1.2 nM (range 0.2 nM-21.9 nM). Pixantrone (BBR-2778) is a novel aza-anthracenedione compound with antitumor activity. What is the genotype of the female parent? Similar trends were observed with 0.5 x and 1 x IC50 of YM155 in combination with Daunorubicin or Cytarabine (data not shown).
MTS assay was performed for Pax3:Foxo1a knockdown mouse aRMS tumor cells treated with DNA damaging agents and microtubule inhibitors. Camptothecin induces apoptosis in cancer cells via microRNA-125b-mediated mitochondrial pathways. Products are for research use only. CI was tested at 0, 100, 200, and 300 nmol/L CI; Nutlin-3A at 0, 2.5, 5, 7.5; and LMB at 0, 2, 8, 32 ng/mL in dosages 0, 1, 2, and 3, respectively. I am sure it can go both ways though, For the best answers, search on this site https://shorturl.im/av09f. Both type I and type II topoisomerases change the linking number of DNA. Phase 2. HT29 cells were exposed to the combination GI80 of gemcitabine (0.2 uM), camptothecin (0.44 uM), cisplatin (68 uM), oxaliplatin (131 uM), doxorubicin (1.2 uM) or etoposide (59 uM) for 18 hours followed by DMSO (-) or 400 nM V158411 (+) for a further 24 hours. Growth suppression by UBE2M silencing is enhanced by DNA damaging agents. There are three main types of topology: supercoiling, knotting and catenation. Clinafloxacin (CI-960, PD127391, AM-1091) is a fluoroquinolone that inhibits both DNA gyrase and topoisomerase IV dually in Streptococcus pneumoniae. http://en.wikipedia.org/wiki/Topoisomerase, http://reverseyourdiabetes.netint.info/?2jbC. Balofloxacin (Q-35) is a quinolone antibiotic, inhibiting the synthesis of bacterial DNA by interference with the enqyme DNA gyrase. You may need to download version 2.0 now from the Chrome Web Store. Growth inhibitory effects of Irinotecan in human pancreatic cancer cells.
If you are at an office or shared network, you can ask the network administrator to run a scan across the network looking for misconfigured or infected devices.
Mitoxantrone inhibits cell proliferative growth of MCF-7/wt cells with IC50 of 0.42 μM.
Genz-644282 is a non-camptothecin topoisomerase I (Topo I) inhibitor that demonstrates potent cytotoxic activity with a median IC50 of 1.2 nM (range 0.2 nM-21.9 nM).