Under the optimal conditions using this enzyme-coupled system (1 U/mL Gox0313 and 4 U/mL NOX-2), 42.32 mM glycolaldehyde was produced from 50 mM ethylene glycol in 8 h with a yield of 83.2%. Among the primary diols, ethylene glycol was efficiently converted to glycolaldehyde by Gox0313 coupled with NADH oxidase-2 (NOX-2) from Lactobacillus brevis ATCC367 for NAD + regeneration. And how thioketal formed from thioethyleneglycol different from ketal . With respect to the superior catalytic activity to primary diols, Gox0313 might be a potentially promising biocatalyst for bioproduction of glycolaldehyde and other hydroxyl aldehydes. Efficient production of glycolaldehyde by Gox0313 and NOX-2 from L. brevis. Find more information about Crossref citation counts. By continuing you agree to the use of cookies. 484.2 mM glycolaldehyde produced from 500 mM ethylene glycol by 120 U/mL Gox0313.
without permission from the American Chemical Society. Find more information on the Altmetric Attention Score and how the score is calculated. Macromolecular Rapid Communications 2019, 40 (12) , 1900057. We use cookies to help provide and enhance our service and tailor content and ads. Copyright © 2014 Elsevier B.V. All rights reserved. Get article recommendations from ACS based on references in your Mendeley library. Please note: If you switch to a different device, you may be asked to login again with only your ACS ID. Under the optimal conditions using this enzyme-coupled system (1 U/mL Gox0313 and 4 U/mL NOX-2), 42.32 mM glycolaldehyde was produced from 50 mM ethylene glycol in 8 h with a yield of 83.2%. Obviously, only one .
Information. Additional NMR and IR spectra as well as fits for the evaluation of reactivity ratios (PDF). Gox0313 selectively oxidize varied diols to hydroxyl aldehydes. Find more information about Crossref citation counts.
But, consider this: Here, we have two carbonyl groups, but only one equivalent of ethylene glycol.
A cytosolic medium-chain alcohol dehydrogenase Gox0313 from Gluconobacter oxydans DSM2003 was heterologously expressed in Escherichia coli BL21 (DE3) and the resulting proteins were purified and characterized. Consequently, these monomers represent promising building blocks for the synthesis of multifunctional PEG for a variety of biomedical purposes. The dense core will not allow for irradiation as stated before.
DOI: 10.1002/marc.201900057. Two novel epoxide monomers 3,3-dimethoxy-propanyl glycidyl ether (DMPGE) and 3,3-dimethoxy-2,2-dimethylpropanyl glycidyl ether (DDPGE) were developed for the introduction of multiple aldehyde functionalities into the poly(ethylene glycol) (PEG) backbone.
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system. Both monomers could be homopolymerized as well as copolymerized randomly with ethylene oxide (EO) in a controlled fashion.
These metrics are regularly updated to reflect usage leading up to the last few days. Myrcenol-Based Monomer for Carbanionic Polymerization: Functional Copolymers with Myrcene and Bio-Based Graft Copolymers. This enzyme could not oxidize sec-alcohols.
This enzyme could not oxidize sec-alcohols.
The recombinant enzyme was confirmed to have a good ability to selectively oxidize the terminal hydroxyl group of varied aliphatic and aromatic diols to the corresponding hydroxyl aldehydes, with no oxidative activities toward hydroxyl aldehydes in the presence of NAD+. Information about how to use the RightsLink permission system can be found at Both Poly(ethylene glycol) and Poly(methyl ethylene phosphate) Guide Oriented Adsorption of Specific Proteins.
To study the distribution of the acetal-protected aldehyde functionalities at the polyether chains, the copolymerization with EO was monitored by in situ1H NMR kinetics experiments for both monomers. Olga Linker, Jan Blankenburg, Kamil Maciol, Matthias Bros. Ester Functional Epoxide Monomers for Random and Gradient Poly(ethylene glycol) Polyelectrolytes with Multiple Carboxylic Acid Moieties. The acetal protecting group for the aldehyde functionality is stable against the harsh, basic conditions of the anionic ring-opening polymerization.